Featured Scientist: Karen Selph

Karen in the lab-van whispering to the flow cytometer!

Hello blog, today Dr. Karen Selph from the University of Hawaii at Manoa shares some of her expert insight into the nutrient-limited world! Karen shared with the blog just a few of the multiple experiments she carried out while on board the NOAA Ship Nancy Foster during NF1704 as part of our NOAA RESTORE project. Karen’s main focus was collecting phytoplankton to analyze with a “flow cytometer” – a machine that tells us how many phytoplankton are out there and gives us a rough idea of their types.

In addition, she measured water column light levels used for photosynthesis by phytoplankton with a photosynthetically-active radiation (PAR) sensor that was attached to the CTD rosette. Karen shared with the blog: "The 6 casts that we performed will yield invaluable information on the light regime during the cruise." 

"The nutrient limiting phytoplankton growth in the Gulf of Mexico is nitrogen.  Nitrogen has many forms, and most phytoplankton can only use reduced inorganic forms (e.g., nitrate (NO₃), ammonium (NH₄)).  However, some phytoplankton can use nitrogen gas (N₂), which is in abundant supply.  This is quite a trick, as the chemistry involved is anaerobic (no or low oxygen) and phytoplankton produce oxygen! 

Karen and Lucy carry out one of the PAR casts

On this cruise, we collected many samples to figure out which of the larger nitrogen-fixing organisms that might be present – in particular, Trichodesmium (usually a bane to those who study zooplankton, as it can clog their nets!!).   Additionally, to assess the base line N-isotopic value for Trichodesmium, we collected samples for stable nitrogen isotope analyses.  We even analyzed a sample of Sargassum to figure out its isotopic signature with respect to the other nitrogen-fixing players in the GOM.  Once we get back to the lab, we will also examine our samples under the microscope looking for the presence of other nitrogen-fixers – some diatoms and other species harbor symbionts capable of this remarkable feat!"

The dynamic duo, Tom and Karen processed hundreds of samples during the survey

Shaun, Karen's travel companion, makes some new friends

Featured guest: Angie Fay Allman

Hi everyone! Today we get to have a unique perspective! One of the crew of the Nancy Foster wanted to stop by and say hello! Meet Angie Fay Ullmann. Angie was our survey technician on the night watch (midnight to noon shift) and helped us safely deploy and recover countless equipment during her watch! She is a NOAA wage mariner, her official position is an “Able-bodied Seaman” and is part of the ‘deck’ department. The science party works closely with the crew members from the survey and deck department as they facilitate multiple and often difficult tasks that include deployment, recovery, set up, break down, winch operations, small boat operations, among many others.

Angie on the bow of the Foster leaving Key West, FL

Angie shared with our blog from her angle:

“I have worked for NOAA for 2.5 years now and have worked on 10 of the 16 ships in our fleet. I am required to do many different tasks on each assignment, but this last cruise really stands out from the rest. I got to work more closely with the scientists and asked more questions about what they were looking for and why. I really enjoyed being more involved and the crew I worked with was a lot of fun and made the days pass by quickly. I hope the data we collected helps contribute to a better future- it gives so much more purpose and satisfaction to my work- even if I don't directly do any science, I'm helping it happen! Before this cruise, I didn't realize the scarcity of bluefin tuna nor that so many scientists try to analyze its behavior in efforts to revitalize its population.”

Angie shows Lucy the ropes, he he

We truly enjoyed working with Angie and hope she will join us again in one of our next adventures on Nancy or another NOAA ship!

Night shift crew!

Featured Scientist: Meet Sydney!

Meet Sydney, who has been an undergraduate student intern at the SEFSC FORCES Lab since Fall 2016! Read about her goals for her first research cruise in her own words...

Sydney models our safety gear

My name is Sydney Harned and I'm a junior at the University of Miami. My goal for this cruise is to learn as much as I can! 

This is my first research cruise, so I'm here to gain some real world experience in the marine biology field. It's been really neat to see scientists specializing in different research areas coming together to work on a project together. 

Sydney is a pro at running the Bongo net!

Since I'm still an undergrad I don't have a specific research focus yet, but I'm hoping to pursue marine genetics in graduate school. For my senior thesis, I'll be using genetics to determine presence/absence of Bluefin Tuna eggs at different stations in the Gulf of Mexico from a previous cruise!

Another Year Complete!

A post-storm sunset captured from the beach in St. Croix, while the Foster (far left) is docked in port

Another cruise is in the books! We can't believe it's over already! This year's cruise was defined by balancing new sampling techniques and research goals with familiar methods and regions.

We made history by becoming the first federal vessel to dock in Havana, Cuba, since the restoration of diplomatic relations between the two countries, but also returned to the familiar U.S. Virgin Islands, where we sampled for the 7th year. We updated our usual pre-determined sampling plan in order to explore the dynamics of ever-changing mesoscale eddies, yet maintained the integrity of historical sampling sites. We welcomed new faces on board, including Cuban observers, and rekindled relationships with colleagues from years past.

Some final stats for Legs 1, 2, and 3 combined:

Sarah and Alexis rinse down the S25 net

• CTD casts = 121
• S10/S25 net tows = 105
• Bongo tows = 41
• Neuston tows = 17
• MOCNESS = 74
• mini-Bongo tows = 66
• drifters deployed = 13
• plankton samples collected = over 500!
• countries visited = 4 (U.S., Cuba, Mexico, Jamaica)
• collaborating countries = 7 (U.S., Mexico, Cuba, Japan, Spain, France, Dominican Republic)
• collaborating institutions = 15

We still have lots of work left to do - unloading the ship, unpacking, cleaning, and repairing all our gear, sorting over 500 plankton samples, identifying larval fish, processing CTD files, running chlorophyll and isotope samples, and more! While we get back to work, check out some of our final images and videos from the last leg of the cruise, and be sure to check back for more next year!

Thanks for reading and sharing in our NF-16-02 adventures!

Leg 3! L-R: Omar, Trika, Sennai, Dan, Alexis, Aras, Mara, Ryan, Alex, Kathryn, Sarah, Adrianne, Angela, LaTreese

Aras patiently filters the mini-Bongo sample through find mesh funnels

Time-lapse: MOCNESS retrieval! 

(Video by Mara Duke)

Time-lapse: S25 tow - this was in very shallow water so you can see the ocean floor!

(Video by Mara Duke)

Adrianne and Angela deploy the CTD while ST Nick directs the winch operator

Ryan takes water samples from the CTD rosette bottles (top), then treats them so the
oxygen concentration can be measured back in the lab (bottom).

Omar (L) rinses the S25 neuston net, while Adrianne and Kathryn hold it steady.
Safety first! Everyone wears hard hats and PFDs, and are tethered to the deck with a safety line.

Day Shift Rules! Mara, Sarah, Sennai, and Alexis take a selfie in their safety gear.

Night Shift makes you crazy! LaTreese, Adrianne, Alex, Omar, Angela, Aars, & Kathryn started work at midnight everyday!

We're done! See you next year!!

Featured Scientist: Catalina "Cati" Mena Oliver

Although we are starting to sample in the eastern Caribbean, today's blog features a 'leg 2' participant: Cati! 

Picoplankton and the microbial food web (from Johnson and Lin, 2009)

Processing plankton samples

Catalina Mena Oliver (Cati) joined us in Cozumel back in May to specifically sample picoplankton during Leg 2. Catalina is a PhD student from the Spanish Institute of Oceanography (Oceanographic Centre of  Baleares - Larval Ecology Group), and she is developing her Doctoral Thesis studying the base of the planktonic food web with special interest in the bacterioplankton communities (picoplankton). 

Dynamic oceanographic currents of the western MED
(Balbin et al 2014)

Picophytoplankton (~0.2 to 2 µm) are the smallest autotrophic organisms on Earth and in stratified oligotrophic regions (like the Mediterranean and the Caribbean) can be a key component as biomass source. During our survey we aim to link the community of primary producers to eddy structures, since these communities can be highly influenced by mesoscale dynamics and by changes in resource supply. The phytoplankton size structure and the relative contribution of the different phytoplankton size fractions in terms of carbon biomass strongly affect the carbon transfer and the ecosystem functioning.

Cati shares her research with the science team

Cati shared with our blog: “I am very grateful to FORCES Lab from SEFSC-NOAA to give me the opportunity to participate in the Nancy Foster survey and I hope we could continue this collaboration in the future. The results obtained will help us to assess if the ecosystem is supported by a microbial food web, dominated by the picophytoplankton fraction, which might be crucial as food source not only for bluefin tuna larval ecology but also for other many species”.
We enjoyed to have Cati aboard and hope that she will sail again with us! We look forward to sharing her results in the future with you our loyal reader! 

Trying on the safety suit! (Cati is a LOBSTER)

The FORCES Awaken

NOAA Ship Nancy Foster alongside in Havana, Cuba

The Nancy Foster is underway once again with an eager compliment of scientists ready to comb the Caribbean for plankton! As with last year the Southeast Fisheries Science Center (SEFSC) is partnering with the Atlantic Oceanographic and Meteorological Laboratory (AOML) to tackle this year’s scientific objectives.

The first part of the 2016 survey (NF-16-02) will be working in waters around western Cuba and east of the Yucatan Peninsula. The focus of this part of the survey will be to continue our study of the distribution and abundance of Atlantic bluefin tuna (ABT) and other tuna larvae in the Gulf of Mexico and western Caribbean Sea. In addition to tuna larvae, the 2016 survey will sample other larval species found near regional coastal reefs including snapper, grouper, parrotfish, and spiny lobster larvae. The goal is to understand the role that the major current systems play in the dispersal/retention of these species and identify possible spawning locations, examine growth and survival of larvae, and increase our understanding of species recruitment to benthic habitat.

The second part of the 2016 survey (NF-16-03) will take place in the vicinity of the Virgin Islands (VI). During this part of the survey, we plan on sampling water properties, currents, and dispersal and transport of fish larvae in the VI and neighboring regions. Results from the survey can enhance our understanding of regional spatial variation in the supply of fish larvae between managed and non-managed areas.

The mighty Neuston net soars again

While the research area and sampling methods may seem similar to last year’s project, we can assure you, our loyal reader, that new and exciting things are in store for 2016! One major change that happened since last year is a rebranding of the very lab that brings you this blog. Say goodbye to the SEFSC’s Early Life History (ELH) Lab and hello to the Fisheries Oceanography for Recruitment, Climate, and Ecosystem Studies (FORCES) Lab! During its humble beginnings, the ELH lab was focused on larval fish taxonomy (i.e. the description and classification of larval fishes) but in the years that followed our work has expanded to a point where now we use tools such as biophysical models, genetics, and isotope analysis to look at the role larval fish and plankton play in the ocean ecosystem in an effort to protect and manage our valuable ocean resources.

The first days of any cruise are indeed hectic as the science party begins to regain their sea legs and shake the rust off. As we put our first nets in the water and deploy our inaugural CTDs north of Havana, Cuba, here is a recap of what the Foster has been up to since we embarked in Miami.

Castillo de los Tres Reyes Magos del Morro

NOAA Ship Nancy Foster arrived at the U.S. Coast Guard Base Miami Beach on May 2^nd, at which point the controlled chaos began. Scientists and crew worked like ants to load all of the necessary equipment and supplies for almost two months at sea; nets, frames, CTDs, hundreds of sample jars, drums of ethanol, drifters, microscopes, computers, liquid nitrogen, fuel, FOOD! Members of the science party flew in from all corners of the world; Mexico, Spain, Japan… Last-minute generator repairs completed…Amongst the chaos we also managed to host a 6^th grade class for a field trip aboard the vessel. Somehow, everything got done and on May 7th, lines were tossed and NF-16-02 and the Nancy Foster were underway! Next stop: Havana.

The day long transit to Havana gave us some time to catch up with each other, (re)familiarize ourselves with the ship, and discuss the upcoming sampling plan. We also took this time to test all of our science equipment. We stopped to deploy our nets and CTD to make sure all of the electronics were functioning properly and went over deployment and recovery procedures (it is a true group effort!).

A Havana icon

The Nancy Foster arrived in Havana on May 8^th to embark the last two members of our science party. The crew and science party enjoyed the prestige of being one of the first U.S. Government vessels to pull in to Havana in a very long time. For more pictures and information on the Nancy Foster's visit to Cuba click here, or here.  With our Cuban colleagues aboard, the Foster left Havana on May 10^th en route to station 001.  Although the weather was less than ideal, we were hoping that our excitement to get the survey started would soothe our seasickness (but if that doesn’t work, there is always sea sickness medication).

Stay tuned as our exciting 2016 survey unfolds. If this is your first Nancy Foster Chronicle, feel free to browse previous years’ blogs to get an idea of what we are all about!

Clyde keeps a watchful eye on the ship